Course Synopsis:

This course focuses on the the recovery, isolation, purification and polishing of products synthesized by biotechnological processes like r-DNA technology, conventional microbial fermentation and enzyme technology. The principles, advantages and limitations of certain purification units also discussed. At the end of this course, students are able to understand the process involved in bio separation and propose a suitable process for different types of product in integration of bio separation schemes.

Course Outcomes:
Ability to analyze process and important parameters involved in recovery and isolation of bioproducts for selected bioseparation units.
Ability to evaluate process and important parameters involved in purification and polishing steps of bioproducts for selected bioseparation units.
Ability to propose bioseparation techniques/processes and RIPP (Recovery, Isolation, Purification and Polishing) scheme.

References:

Harrison, R.G. Todd, P., Rudge S.R. and Petrides D.P. 2003. Bioseparations Science and Engineering, Oxford University Press.
Rajni Hatti-Kaul et al. 2003. Isolation and Purification of proteins (Biotechnology and Bioprocessing), Marcel Dekker Ltd.
Sabramanian Ganapathy. 1998. Bioseparation and Bioprocessing. A handbook, 2nd Edition, Wiley.
Paul, A. Belter, Clussler, E.L. and Wei-Shou Hu. 1998. Bioseparations: Downstream Processing for Biotechnology, Wiley.
Michael S. Verral. 1996. Downstream Processing of Natural Products, A Practical Handbook, Wiley.
Sivasankar, B. 2006. Bioseparations: Principles and Technique, Prentice Hall.
Ladisch, M. R. 2001. Bioseparations Engineering: Principles, Practice and Economics, Wiley- Interscience.